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Indeed, the conversion of a catalytic Cys thiol into a disulfide is a powerful means for shutting down enzymatic activity because disulfides are poor nucleophiles compared to thiolates

Am J Psychiatr 2011;168:90412

Louis This article is republished from The Conversation under a Creative Commons license

5e), but to determine the persistence of agonistreceptor complexes, we adapted our TR-FRET binding assay to include reversible labeling using cleavable SNAP-biotin complexed to streptavidin-terbium to label the surface GLP-1Rs and, after internalization with different FITC-agonists, stripping residual surface receptors of label to ensure that FRET was derived only from internalized receptors (see Fig