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The proton that it delivers has been previously scrubbed of deuterium through the action of isomerases to exchange deuterium with hydrogen in the cytoplasmic water and techniques exploited by flavoproteins such as proton tunneling to support a high deuterium kinetic isotope effect (KIE) ( Cytochrome P450 enzymes (CYPs), primarily localized to the endoplasmic reticulum or the mitochondria, oxidize a variety of different substrates, replacing a proton with a hydroxyl group in the substrate, splitting molecular oxygen, and producing water as the other product

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