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For immunohistochemistry, the sections were deparaffinized and rehydrated, processed with microwave antigen retrieval, and incubated overnight with primary antibodies against Ki67 (ab15580), and CD8 alpha (ab217344) (Abcam, Cambridge, UK) at 4 C

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3 Considering lab work thyroid, blood sugar/insulin, and hormone markers can reveal contributing factors that diet and medication alone wont fix

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