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In brief, male mice were deeply anaesthetized with an intraperitoneal injection of 7% chloral hydrate and transcardially perfused with a modified ice-cold artificial cerebrospinal fluid (aCSF) (described below)

The seed culture was transferred into a sterilized 5-L bioreactor (Shanghai Bailun Bio-Technology Co., China) at a 1:10 (v/v) inoculation ratio

Individual variation is significant, with factors like age, baseline condition, and protocol adherence affecting timelines

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To quantify the relationship between weight loss and blood pressure reduction could help clinicians to refine treatment targets and better understand the cardiovascular effects of contemporary anti-obesity medicines