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The levels of lipid ROS were analyzed through FL1/FL2 chanel using a Becton Dickinson FACS Calibur machine, and quantitation of lipid peroxidation was achieved by normalizing FL1 to FL2 channel in FlowJo V10 software

27 Microbial characteristics of MASLD/NAFLD Gut flora dysbiosis in MASLD has distinct characteristics closely linked to obesity, insulin resistance, and hepatic steatosis

Vitamin B12 enhances the metabolic function of the cells helping to boost energy release and support overall wellbeing

Aliquots (~0.1 mg) of the target compounds were dissolved in methanolic KOH solution (1.2 mol/L, 80% MeOH), incubated at 50 C for 2 h, then adjusted to pH 5.0 and analyzed by means of UPLC-TOF MS in the negative electrospray mode

It binds to ghrelin receptors located in both the hypothalamus and pituitary gland, initiating a natural pulsatile secretion pattern of growth hormone