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Metabolomics and mass spectrometry imaging reveal channeled de novo purine synthesis in cells

CYP2E1 is also involved in the oxidation of compounds such as benzene and acetone, and it exacerbates oxidative stress in hepatocytes by generating reactive oxygen species (ROS)

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The principle of this assay is that DCFH-DA diffuses through the cell membrane and is enzymatically hydrolyzed by intracellular esterases to nonfluorescent dichlorofluorescin (DCFH)

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