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However, it must be noted that the PGN administration in drinking water was only for 2 weeks

While certain methods employing laborious pre-column chemical derivatization combined with fluorescence or MS/MS detection can reach lower limits in the fmol/L or pmol/L range [57, 69], they usually compromise on the scope of analyzed compounds and analysis throughput

This suggests that non-responders were less severely affected by ME/CFS at baseline, and that the main positive effect of nasal B12 administration occurs in more severely affected patients