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Chemiluminescent signal was read using a ChemiDoc system (BioRad #1708265), and the expression of bands of the target protein for each animal was expressed as % of -actin loading control

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We encourage you to report any unusual symptoms, such as nausea, vomiting, or diarrhea, and provide support to manage these effects

Pdcd4 was initially demonstrated to preferentially inhibit translation of the luciferase mRNA possessing an artificial stem-loop structure at 5UTR with the free energy of 44.8 kcal/mol