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After an incubation time of 60 min, quantitative substrate and NADH conversion with equimolar MSA production was verified by absorption spectroscopy in combination with the MBTH-based aldehyde quantification method

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UCB causes mitochondrial ROS production, which consequently causes activating p38 and p53 downstream, ultimately leading to the increased regulation of the pro-apoptotic protein Bax and the decreased regulation of the anti-apoptotic protein Bcl-2 and phosphorylation of Bcl-xL/Bcl-2 associated death promoter (Bad) (83), thus causing apoptosis