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In Arabidopsis , among the upregulated GSTs, which were induced by different wavelength and light intensities, AtGSTU17 and AtGSTU5 share some common light-responsive elements (ATC-motif, Box 4, G-box, and LAMPelement)
A potential direct metabolic impact of SUN1 H118Y was tested in human hepatoma (Huh7 and HepG2) cells using semi-quantitative lipid staining (BODIPY 493/593) and determination of insulin-stimulated AKT phosphorylation via immunoblot, as well as transcriptional analysis (qPCR) of lipid-related gene expression
Typically, top biomarker candidates identified from screening studies will be validated through a combination of secondary focused screens and individual gene perturbation studies which include independent sgRNAs, rescue experiments, and functional assayssuch as proliferation, apoptosis, or immune cell co-cultureto confirm their role in tumor biology
