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Glucose/galactose adapted cells were cultured in DMEM containing 10% dFBS, 100 U ml 1 penicillin and 100 g ml 1 streptomycin, 1 mM sodium pyruvate and 25 mM glucose (Sigma-Aldrich, G7021-1KG) or 25 mM galactose (Sigma-Aldrich, G5388-500G) for a minimum of 1 month before being used experimentally

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Sci Rep 11(1):1 Maynard S, Hall A, Galanos P, Rizza S, Yamamoto T, Gram HH, Munk SH, Shoaib M, Srensen CS, Bohr VA, Lerdrup M (2022) Lamin A/C impairments cause mitochondrial dysfunction by attenuating PGC1 and the NAMPT-NAD+ pathway

Furthermore, these findings change our perspectives about GSH biology and the acquisition of amino acids by cells

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