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BSO treatment was performed similarly as for ITCs by adding it (100 mM stock solution prepared in water) to the medium (either on its own or in combination with ITC) to obtain BSO concentrations ranging from 50 M to 700 M

This may help explain why it is little used by health professionals

It is clear from the studies going back many decades that ascorbate and glutathione can be redox-coupled

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These mediators link regional dysbiosis to distal organ dysfunction, underpinning the inter-organ axes described throughout this review that redefine our understanding of disorders like mastitis, ketosis, and BRD