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Finally, coverslips were washed 3 times with 1 PBS and mounted using Vectashield containing DAPI (Vector Laboratories, H-1200-10)

Glutathione plummets in the lungs even due to activities that we often dont associate with the lungs

doi: 10.1186/s12885-018-5030-1 124 PinheiroLCLPereiraRFrancelinoALGuembarovskiAFMLFugantiPEde OliveiraKBet al

Flow cytometry analysis was performed using FSC/SSC gating to identify single cells and exclude debris, followed by SSC-A versus SSC-H gating to remove doublets