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Measurements were validated using the Wave software (Agilent, Santa Clara, California, USA) and normalized to DNA concentrations per well, analyzed in stained cells (Hoechst 33342) using a plate reader (Tecan Infinite M200, Tecan Group Ltd., Mnnedorf, Switzerland) with an excitation wavelength of 350 nm and an emission wavelength of 461 nm

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Might a biopsy give us clearer answers or help rule out other conditions