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35 Geographic and cultural factors also influence the prevalence of DN

Needle wash was performed between samples using dichloromethane: isopropanol: acetonitrile at 1: 1: 1

Materials and Methods Bacterial Strains, Recombinant DNA Work, Media, and Growth Conditions A complete list of the bacterial strains and plasmids is given in Supplementary Table 2

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Water retention or mild bloating: Small amounts of fluid retention can occur when starting peptide analogues of growth hormone because growth hormone causes sodium and water retention in the tissues, resulting in slight puffiness or bloating