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Endocrine 71 , 104112 (2021)

Next, the plates were washed five times with PBST before the addition of 20 L/well of 2 g/mL anti-Penta-His-HRP (MCA5995P, clone ABD2.2.20, Bio-Rad) detection, prepared in HISPEC assay diluent (Bio-Rad)

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