(PubMed) Liang WC, Ohkuma A, Hayashi YK, et al
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10.1371/journal.pone.0145890 PLoS One 107 McCulloughA
Stracke H, Hammes HP, Werkmann D, Mavrakis K, Bitsch I, Netzel M, Geyer J, Kopcke W, Sauerland C, Bretzel RG, et al: Efficacy of benfotiamine versus thiamine on function and glycation products of peripheral nerves in diabetic rats
The standard clinical recommendation is to hold for at least 90 to 120 seconds