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At the completion of the incubation, the cells were washed three times with ice-cold phosphate-buffered saline (PBS), and then, the fluorescence was measured by fluorescence microscopy at an emission wavelength of 460 nm using an excitation wavelength of 360 nm for the DCF fluorophore

Methods Reagents and tools table Cell lines and cell culture HEK293T, HEK293T RSMC heterozygous knockout (RSMC +/ ), HEK293T Flag-Sororin, HeLa, dCad9- GFP 14 -integrated HeLa, HeLa RSMC-3Flag, HeLa PARP1 WT and KO (provided by X Xu) cells were grown in DMEM (Gibco,11995500BT) supplemented with 10% FBS (ABW, AB-FBS0500) and antibiotics

doi: 10.1007/s10482-016-0737-y 71 JoshiS.McMackenM.Kalantar-ZadehK

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Open Ophthalmol J 6:7578 Kiyota N et al (2024) The effect of -blocker eye drops on pulse rate, ocular blood flow, and glaucoma progression: a retrospective longitudinal study