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"The role of hepcidin, ferroportin, HCP1, and DMT1 protein in iron absorption in the human digestive tract"

SPECT (Single Photon Emission Computed Tomography): Visualizes areas of hypoperfusion (low blood flow) that correlate with specific cognitive deficits

After 48 h, luciferase activity was measured using a Dual-Luciferase Reporter Gene Assay Kit (Beyotime), according to the manufacturers instructions

1 mL of sulfanilamide (10 mM/L) was added to 100 L of sample, standard, or blank and mixed thoroughly before standing for 5 min before adding 100 L of 1 mM/L NEDA to the samples and standard, mixed thoroughly, and stood for another 5 min

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