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G4251) in 150 mM NaCl, 10 mM Tris pH 7.4, 1 mM EDTA, 10 mM -mercaptoethanol and 1 mM PMSF]

Risks appear particularly elevated in individuals with comorbid depression or BED, where long-term outcomesespecially following discontinuation of pharmacotherapyremain insufficiently studied [143]

Detection of intracellular ROS ROS was measured using the fluorescent probe 2,7-dichlorofluorescin diacetate (DCFH-DA, Sigma), which can be hydrolyzed to DCFH within cells

Notably, the paucity of SGLT2i studies prevented the analysis of clinical outcomes for this drug class independently