The medium comprised Lebovitz L-15 base medium with 8.3% fetal bovine serum (FBS-Biowest), 8.3% Tryptose Phosphate Broth (Sigma-Aldrich), hydrocortisone (Sigma-Aldrich), ascorbic acid (Sigma-Aldrich), Glutamax (Life Technologies), 100 nM dexamethasone (Sigma-Aldrich), and 100 nM N -hexanoic-Tyr, Ile-6 aminohexanoic amide (Dihexa) (Active Peptide)
Dihexa reduces pro-inflammatory IL-1beta and TNF-alpha while increasing the anti-inflammatory cytokine IL-10
This window is the clinical foundation of the stack
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