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Both dethymoquinonated and regular volatile oil from black seed lowered arterial BP and HR in rats, with the CV depressant effects potentially mediated by central mechanisms involving activation of 5-hydroxytryptaminergic and muscarinic pathways (425, 426)

Recently, despite administration of NAC 600 mg po tid, a sustained increase in GSH concen-trations could not be found in the plasma, bronchoalveolar lavage fluid, or lung tissue of patients with chronic obstructive pulmonary disease

Sigma-Aldrich, Munich, Germany) (1:1 v/v), vortexed and centrifuged at 28,000 g for 5 min at 4C