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JOP 11: 99-112, 2010

10.3923/ajpp.2010.338.349 32 PreussM

The analysis of the optimized structures of the MichaelisMenten complex indicate that the polar residues at position 13 (Cys or Ser) stabilize the deprotonated GS , which in turn acts as the catalytic Brnsted base and abstracts the proton at position C2 of substrate 7 (Extended Data Fig