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The vagus nerve is considered a vital channel for two-way communication between the brain and gut, which can differentiate between pathogenic and non-pathogenic bacteria [96]

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Synthesis of cDNA was performed with 1 g of total RNA from each sample using Quantiscript Reverse Transcriptase, Quantiscript RT-buffer and RT Primenr-mix of QuantiTect Reverse Transcription kit (Qiagen GmbH, Hilden, Germany) according to the manufacturers protocol

In the Danish Prospective Diet, Cancer, and Health prospective study that followed over 57,000 men and women for 14 years, the risk of rectal cancer was found to be 42% higher in current smokers compared to nonsmokers