At 7 d the media was aspirated, and cells were incubated at 4C with 15 ml cold PBS (Gibco, 10010-023) for 10 min
That's what you have to expect
Where exactly to inject GLP-1 in the thigh
Hippophae Rhamnoides Water, Water, Diisopropyl Sebacate, Titanium Dioxide (CI 77891), Propanediol, Butylene Glycol, Butyloctyl Salicylate, Dibutyl Adipate, Ethylhexyl Triazone, Terephthalylidene Dicamphor Sulfonic Acid, Niacinamide, Alcohol Denat., 1,2-Hexanediol, Tromethamine, Dimethicone, Hippophae Rhamnoides Oil, Caprylyl Methicone, Diethylamino Hydroxybenzoyl Hexyl Benzoate, Polymethylsilsesquioxane, Polysilicone-15, Isododecane, Octyldodecanol, Bis-Ethylhexyloxyphenol Methoxyphenyl Triazine, Pentylene Glycol, VP/Eicosene Copolymer, Polyglyceryl-3 Distearate, Potassium Cetyl Phosphate, Cetearyl Alcohol, Glyceryl Stearate, Dimethicone/Vinyl Dimethicone Crosspolymer, Polyglyceryl-4 Isostearate, Hydroxyacetophenone, Methylpropanediol, Polyester-5, Isopropyl Titanium Trisostearate, Aluminum Hydroxide, Polyglyceryl-10 Myristate, Carbomer, Glyceryl Stearate Citrate, Adenosine, Hydroxypropyl Methylcellulose Stearoxy Ether, Sodium Polyacryloyldimethyl Taurate, Xanthan Gum, Glutathione, Polyether-1, Cetyl Hydroxyethylcellulose, Ethylhexylglycerin, Xyloglucan, Arginine, Alpha-Arbutin, Ascorbic Acid, Cyanocobalamin, Panthenol, Tocopherol, Ubiquinone, Hydroxypropyl Cyclodextrin, Glycerin, Polyglyceryl-10 Laurate, Thioctic Acid, Hydrogenated Lecithin, Tremella Fuciformis (Mushroom) Extract, Potassium Hydroxide
One more note: if you're pregnant, high doses of Vitamin A can be a concern, so a lot of people skip topical retinoids (including Retinyl Palmitate) during pregnancy just to be safe
