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For purification, a cell pellet was resuspended in lysis buffer containing 50 mM Tris pH 7.5, 500 mM NaCl, 50 ug/ml DNase1, 50 ug/m lysozyme, 0.5 mM Benzamidine, 0.5 mM PMSF, and EDTA Free protease inhibitor cocktail (Roche), sonicated and debris removed by a 45-min centrifugation at 50,000 x g

PMID 25616211

Nevertheless, both dietary intakes of vitamin B12 and serum concentrations of vitamin B12 were unable to observe significant associations between NAFLD patients and controls in 317 Iranians (44) and 54 participants in Greece (15), respectively

A recent study showed that a glycocalyx-mimetic nanoparticle library may facilitate drug delivery [118]