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Metastasis Studies Breast Cancer Model Validation of shRNA Target Genes Cell lines derived from 4T1 metastatic lung tumors (see above) were stably transduced with pLKO.1 vectors (Control and pooled shRNAs targeting Gstt1 ), harvested by trypsinization, washed twice in PBS, resuspended at 1 x 10 4 in 100ul PBS and injected into the venus sinus of BALB/c mice (Jackson Laboratory) to establish lung metastases

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