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Full-stomach precautions may include rapid sequence induction when appropriate, airway-protective strategies, suction readiness, careful positioning, and consideration of awake intubation in selected difficult-airway/high-aspiration-risk scenarios

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For the brain fractionation as per The Thermo Scientific Pierce bicinchoninic acid Protein Assay Kit was used to quantify proteins in the tissue homogenates analyzed

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Three modules are GPI-anchored protein signal sequences from DAF, BY55, and CEAM7, and the other three are transmembrane domains (TMDs) from ITAV, ITA5, and ITB3 as used previously (Yang et al., 2022)

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