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An equal number of sex-, age-, site of onset-, disease-duration-matched untreated patients with ALS were analysed as a control group

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Adapted from the Society of Clinical Research Associates 2 Impact of Usability Issues on Clinical Trial Data Conducting HF validation tests before major clinical trials, as recommended by the FDA, supports more reliable trial outcomes by identifying usability issues early in development

(2022b), where a reaction solution with a total volume of 2.1 ml consisted of 0.1 ml 0.06% H 2 O 2 , 1.7 ml of 50 mmol/L potassium phosphate buffer containing 0.1 mmol/L EDTA-Na 2 , 0.1 ml of 6 mmol/L ASC, and 0.1 ml supernatant