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Standard curve of GSH and GSSG The standards of GSH and GSSG were prepared and diluted in extraction buffer (5% sulfosalicylic acid, 5 mM EDTA): MES buffer (0.4 M 2-(N-morpholino) ethanesulphoric acid, 0.1 M sodium phosphate, 2 mM EDTA, pH 6.5) = 1:15 or 1:7 respectively to provide 10 concentrations in the rage of 0.5 32 M

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Diabetes 61 , 145154 (2012)

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