Available from: J L Sievenpiper 1 2 3 , J Ard 4 , M Blher 5 , W Chen 6 , J B Dixon 7 , A Fitch 8 , L Gigliotti 9 , K Khunti 10 , A Lecube 11 12 , M E J Lean 13 , B Mittendorfer 14 , A F H Pfeiffer 15 , D H Ryan 16 , T Vilsbll 17 18 , L F Van Gaal 19
The G protein-coupled receptor of GLP-1 (GLP-1R) originally identified in islet -cells is widely expressed in extrapancreatic tissues as well including the lungs, kidney, brain, the enteric and peripheral nervous system, lymphocytes, smooth muscle cells (SMCs), and atrial cardiomyocytes [32] The specific identity of GLP-1 receptor-positive (GLP-1R+) cells in the heart remains uncertain
After washing five times with lysis buffer, the complexes were mixed with lysis buffer containing 290 mM imidazole, and the bound proteins were eluted by boiling in 5 sodium dodecyl sulfate (SDS) loading buffer for 5 min
232,233,241,259 (Fig
doi: 10.1056/NEJMoa0806470 49 Association AD