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After washing with PBS another three times, the cells were permeabilized with 0.5% Triton X-100 for 15 min and washed with PBS repeatedly

Causes While causes of carnitine deficiency is unknown, it is believed some dog breeds show an increased risk of developing the deficiency, including Boxers, Doberman Pinschers, Great Danes, Irish wolfhounds, and other giant breeds

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10.1093/carcin/bgy038 31 SassoliC.PiniA.ChelliniF.MazzantiB.NistriS.NosiD.et al (2012)