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Severe heart failure patients may not handle the fluid volume well

Diabetes 63 , 37213733 (2014)

(131) proved the neuroprotective efficacy of intracerebroventricular injection (i.c.v.) of Ex-4 exerted after transient ischemia/reperfusion in rats and first suggested that Ex-4 played a neuroprotective role probably by stimulating the GLP-1R/-endorphin signal pathway in hippocampal microglia (76)

Afterwards, the supernatants were collected and the fragments snapped frozen prior to homogenization and protein quantification with the Pierce BCA Protein Assay Kit (Prod #23225, ThermoFisher Scientific, France)