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genetic associations with other risk factors were obtained from summary statistics in the Neale Lab GWAS of the UK Biobank, controlling for age, age 2 , and 20 principal components (Additional file 1: Table S1)

mRNA isolation and RT-PCR RNA extraction was carried out using the PureLink RNA minikit (Ambion) according to the manufacturers protocol 20

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however, this study did not assess NAD + levels or mitochondrial function 328

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