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After 2 h incubation, cells were washed twice with cold PBS and were extracted with 80% ice-cold methanol at 2 10 6 cells/mL

Ferroptosis as a target for protection against cardiomyopathy

The study selected 2.5 mM DTNB for further experiments

For antibody staining, antigen retrieval was performed on the slides by incubating them in a steamer for 40 min in citrate buffer (Vector Labs, H-3300-250)

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