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Sequencing libraries were prepared using the SMARTer Stranded RNA-Seq Kit (Clontech Laboratories, Inc., USA) and the Poly(A) RNA Selection Kit (LEXOGEN, Inc., Austria) by the manufacturers recommendation

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Administration of a pharmacological dose of recombinant human ghrelin increased proximal gastric tone through central and peripheral effects ( A synthetic pentapeptide ghrelin receptor agonist, relamorelin, is 15130 times more potent than natural ghrelin (Figure 1 in patients with either type 1 or type 2 diabetes mellitus with prior documentation of delayed gastric emptying (Figure 2 ( FIGURE 1 FIGURE 2 Motilin Receptor Agonists The most common motilin receptor agonists are the macrolide antibiotics that stimulate gastrointestinal motilin receptors, especially gastric receptors