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Saponin at 100 g/ml was used as a positive control for 100% cell lysis, blood (1% hematocrit) with DPBS as a negative control for no cell lysis, and DPBS as a blank

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Establishment and cultivation of PDCOs PDCOs are typically established by enzymatically or mechanically dissociating tumor tissues into single cells or small clusters, which are then embedded in a supportive extracellular matrix such as Matrigel [30, 31]