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DDX3X rather appears to bridge the outgoing capsids to the NEC directly on the nuclear membrane by virtue of its interactions with the NEC and the multiple capsid/tegument components identified by MS as DDX3X binding partners

For pbgst-ko 2A and 2B plasmids, the 5 and 3 targeting regions of the pbgst gene were PCR-amplified using the corresponding primer pairs that produced DNA fragments as detailed in Supplementary Table S2

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