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Activation of viscerosensory afferents to the NTS with systemic CCK To test whether leptin alters the activity of PPG neurons and their downstream targets in response to a visceral stimulus, a previously validated Fos assay was adapted (Maniscalco and Rinaman, 2013)

GHK-Cu is one of the very few compounds that stimulates production across virtually every component of the extracellular matrix simultaneously