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Despite extensive in vitro (cell culture) and gene expression data, there are relatively few large-scale, randomized controlled trials on topical GHK-Cu for anti-aging in human skin

Put plainly, the off period is not there because the compound stops working it is there because no one can point to human data showing that running it indefinitely is safe, and a scheduled break is the honest way to respect that gap

doi: 10.4161/gmic.25487 47 GuY.Bartolom-CasadoR.XuC.BertocchiA.JanneyA.HeubergerC.et al

Malondialdehyde (MDA), a naturally occurring product of lipid peroxidation and an indicator of oxidative stress, was measured with ELISA using TBARS Assay (Cayman Chemical Company, Ann Arbor, MI, USA)

Safety: There is insufficient evidence regarding safety in humans