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doi: 10.3389/fmicb.2025.1527480 Received 13 November 2024 Accepted 24 January 2025 Published 07 February 2025 Volume 16 - 2025 Edited by Ming Jiang, Guangdong Academy of Agricultural Sciences, China Reviewed by Alvaro Mourenza Flrez, University of Southern California, United States Xianliang Zhao, Henan Normal University, China Updates Copyright 2025 Du, Wu, Zhu, Yang, Zhao and Fang

In many experimental animal models, MTX has been found to increase serum urea and creatinine levels (Reference Moodi, Hosseini and Abedini16,Reference Tousson, Zaki and Abu-Shaeir50,Reference Jafaripour, Naserzadeh and Alizamani51) , but there are also studies showing that MTX reduces serum urea concentration (Reference Budancamanak, Kanter and Demirel52Reference Severin, Campagno and Brandoni54)