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Figure 2 Interestingly, many of the initial studies elucidating NRF2-ARE-mediated cellular protection utilized tert -butyl hydroquinone (tBHQ), a synthetic antioxidant and inducer of the NRF2 pathway via its auto-oxidized, electrophilic form tert-butyl benzoquinone (tBQ) (Rushmore and Pickett, 1990

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Tissue Sampling Preparation (homogenate) The 4 rat's livers were extracted from each group, washed with phosphate-buffered saline (PBS PH 7.4), and then dried with filter paper before measuring the weight of each liver

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