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Dissolved Oxygen (DO): Oxygen is essential for the aerobic microorganisms within the biofilm

Thus, while up to 70% of PNA mice displayed irregular cyclicity, characterized by the appearance of only one generation of (mainly persistent regressing) corpora lutea, 100% of PNA mice treated with metformin showed regular ovarian cyclicity, denoted by the presence of two generations of corpora lutea, as a sign of preserved regular ovulations (Fig