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J Cell Sci 115:20532065 Petit FM, Serres C, Bourgeon F et al (2013) Identification of sperm head proteins involved in zona pellucida binding

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In the previous study, we had successfully generated FGF5 -knockout heterozygous sheep using the CRISPR/Cas9 system by direct one-step cytoplasmic injection of Cas9 mRNA and sgRNA into zygotes, and the relationship between FGF5 gene and wool length in sheep was proved 22