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Samples were reconstituted in 90 l of 50% acetonitrile (Fisher Scientific, A955) and transferred to glass vials for LCMS analysis

The crotonylation of H3K9 was significantly enhanced by NaBu but suppressed by ACADS knockout, revealing that ACADS regulates histone 3-lysine 9-crotonylation (H3K9cr) [45]

This feature makes them highly reactive

Analytical methods such as principal component analysis (PCA) and machine learning (supervised and unsupervised) stratify tumors into aggressive and non-aggressive and decipher novel biological insights (277)

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