coli BL21(DE3) containing plasmid pANT43 was inoculated from an overnight preculture at a 1:100 ratio in four 2 l baffled Erlenmeyer flasks filled with 400 ml TB medium containing 50 g ml 1 kanamycin sulfate and incubated at 37 C, 140 rpm using a 5 cm shaking diameter for 2.75 h
10.1161/01.cir.102.16.1931 51 MinnitiC
The primary antibodies used in the immunohistochemistry included: rabbit anti-S100 (1:1000, Abcam ab41548), mouse anti-S100 (1:500, Sigma S2532), guinea pig anti-GABA (1:200, Millipore ab175), rabbit anti-MAOB (1:200, Abcam ab175136), chicken anti-GFAP (1:1500, Abcam ab4647), rabbit anti-ABAT (1:500, Abcam ab108249), and rabbit anti-putrescine (1:200, MyBiosource MBS2090435)
Venom proteome of the monophagous spider Ammoxenus amphalodes: a potential source of termite-specific toxins
With this biotransformation, liver detoxification, a long-standing barrier in nanomedicine translation, can be turned into a bridge toward maximizing targeting and minimizing nanotoxicity
