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This work was supported by the National Natural Science Foundation of China (grant numbers 82202989, 82573498)

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For the analysis of deglutamylation activities toward -glutamyl- S -allyl-L-cysteine and -glutamyl- S -allyl-L-cysteine sulfoxide, the enzyme assay reaction mixture consisted of 0.67 g l -1 yeast crude protein, 50 mM Tris-HCl (pH 8.0), 10 mM glycylglycine, and 1 mM -glutamyl- S -allyl-L-cysteine or -glutamyl- S -allyl-L-cysteine sulfoxide was incubated for 6 h at 37C