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Saneyoshi T, Wayman G, Fortin D, Davare M, Hoshi N, Nozaki N, et al

MS2 spectra were fragmented by higher energy collision dissociation (HCD) (normalised collision energy (NCE) = 30) activation mode, and the ion-trap was selected as the mass analyser

They are typically akin to fragments of protein molecules and are typically around 14 plus amino acids long with some variations

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In the female cohort, 50% of the metabolites were lower (FC 1.1) at D1PRE, constantly increasing through our timeline to reach 35% at D2POST