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Cells 183, 10431057.e15
The positive health impacts seen in people with type 2 diabetes who take metformin, coupled with fascinating findings from animal studies, have sparked an intriguing question: could metformin be the key to unlocking longer, healthier lives?
doi: 10.2105/AJPH.2015.302773
The resulting cells were fixed in 4% paraformaldehyde and incubated with a blocking buffer for 1 h, and the cells were permeabilized with 0.2% Triton and 0.1% Tween 20 in a blocking buffer (3% bovine serum albumin in PBS) for 2 h
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