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Wild-type (ECACC) and -arrestin-less HEK293 cells 41 stably expressing SNAP-GLP-1R were generated by G418 selection and maintained in DMEM at 25 mM d-glucose supplemented with 10% FBS and 1% penicillin/streptomycin

Membranes were blocked for 1 h in 5% non-fat dry milk prepared in 1 Tris-buffered saline containing 0.1% Tween-20 (TBST)

Fras JP, et al

2023;78:S8112

Researchers at institutions including the University of Gothenburg and Scripps Research Institute showed that semaglutide and similar compounds reduce voluntary alcohol intake, decrease nicotine self-administration, diminish cocaine-seeking behavior, and prevent relapse-like drinking after periods of abstinence